In Vivo Neuropharmacology and Neurophysiology

Pharmaco-Based fMRI and Neurophysiology in Non-Human Primates
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Synaptic Transmission 4. Signal Transcuction in the Brain 2.

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This volume presents classical approaches to in vivo neuropharmacology and neurophysiology, such as c-fos, electrochemistry, microdialysis microstimulation, . See reviews and reviewers from In Vivo Neuropharmacology and Neurophysiology.

Monoamines, Acetylcholine, and Orexin 7. Neuropeptides 8. Autonomic Nervous System Neural and Neuroendocrine Control of Internal Mileau Pain Neuroinflammation Sleep and Arousal Cognitive Function and Behavior Mood and Emotion Marsh, Donald J Frank L. Day Professor Emeritus of Biology.

Marshall, John Professor of Medical Science. This line of research combines cutting-edge in vivo and in vitro electrophysiology, neuropharmacology, multi-electrode interfaces and neuroprosthetics, and behavioural neuroscience. As part of this collaboration, Giuliano Taccola explores new pharmacological and electrical stimulating protocols to restore motor functions in preclinical rodent models of spinal cord injury.

Lecture 2 Neurophysiology

In the coming years, in vivo electrophysiological and behavioural assessments in preclinical models will be associated to in vitro recordings from reduced spinal cord preparations in order to:. Further define the biophysical membrane properties of motoneurons during development, the organization of neonatal interneuronal spinal networks generating rhythmic activity and their pharmacological modulation.

Research Interests

Explore the effects of passive training on spinal circuits, also comparing real locomotor training with a recently invented robotic device BIKE, Bicycle Induced Kinetic Exercise that reproduces a standardized hindlimb passive motion in the isolated spinal cord — leg attached preparation of neonatal rats. Limit the acute consequences of a spinal cord injury and support the recovery of functions in chronic spinal cord injuries, by associating innovative electro-stimulating protocols, stochastically modulated in both frequency and amplitude noisy , with the design of innovative multi-electrode epidural interfaces for independent multi-site stimulation and recordings, and combine them with new pharmacological interventions.

The research interests of the laboratory are focused on the neurophysiology and neuropharmacology of the spinal cord, before and after lesion, with a particular emphasis on the functional organization of the neuronal circuits responsible for the generation of the locomotor rhythm locomotor central pattern generator, CPG. The main scientific goal is to identify new experimental strategies to activate the CPG, in a perspective to propose new therapeutic interventions for the functional recovery of standing posture and deambulation after spinal cord injury.

From molecules… to behavior

Several innovative patterns of electrical stimulation have been devised in our lab by sampling the rhythmic noisy waves that appear during fictive or real motor patterns recorded, respectively, from the isolated neonatal rat spinal cord or from EMG recordings of lower limbs during real locomotion in healthy volunteers. Their combination with pharmacological stimulation is a further aspect that our group is considering to synergize the performance of these protocol.

In collaboration with international partners, experts in preclinical rodent models, we are also assessing how different protocols of electrical stimulation or forced passive motor activity can modulate the hyperactivity of in vitro dorsal networks and thus alleviate the neuropathic pain associated with a spinal damage or with a lesion of the peripheral nerve.

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